F(ab')2 Goat anti-Mouse IgM Heavy Chain Antibody Affinity Purified

Specifications

Production & Epitope

Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species.

Applications

Not all listed applications have been specifically tested by our laboratory.