Bethyl Laboratories
Mouse anti-Skeletal Troponin I Monoclonal Antibody [FI-23]
Primary
Catalog #A500-047A
TargetSkeletal Troponin I
HostMouse
CloneFI-23
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. The Troponin I subfamily contains three genes: Troponin I-skeletal-fast-twitch, Troponin I-skeletal-slow-twitch, and Troponin I-cardiac. Troponin I-cardiac is exclusively expressed in cardiac muscle tissues. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19429].
Specifications
Clonality
Monoclonal
Clone
FI-23
Host
Mouse
Immunogen
Human Skeletal Troponin I
Isotype
IgG1
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetSkeletal Troponin I
Antigen Species
Human
Gene ID7136
Gene Symbol
TNNI2
Gene Name
troponin I2, fast skeletal type
Uniprot IDP48788
Protein Name
Troponin I, fast skeletal muscle
Product Information
Applications
IHC, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Monoclonal antibody was purified from cell culture supernatant.
Immunogen was human Skeletal Troponin I
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Western Blot (WB)1:1,000
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A500-047A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-047A · Lot 1 (current lot)Open
Safety Data SheetPDF · A500-047AOpen
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Resources
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