Bethyl Laboratories
Goat anti-Mouse Kappa Light Chain Antibody Affinity Purified
Secondary
Catalog #A90-119A
TargetMouse Kappa Light Chain
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
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Product Description
The kappa light chain is a component of an Ig molecule that typically forms an Ig molecule linking to a heavy chain. The human body makes both kappa and lambda light chains, and elevated kappa levels may suggest disorders such as amyloidosis or multiple myeloma.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chain common to all mouse immunoglobulins. Less than 1% cross-reactivity was detected against lambda light chain and non-immunoglobulin serum proteins. This antibody may cross react with kappa from other species. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A90-119AOpen
Product DatasheetPDF · A90-119A · Lot 260414 (current lot)Open
Safety Data SheetPDF · A90-119AOpen
Product DatasheetPDF · A90-119A · Lot 16Open
Safety Data SheetPDF · A90-119AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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