Bethyl Laboratories
Mouse anti-Cytokeratin 14 Recombinant Monoclonal Antibody [LL002]
Primary
Catalog #A700-293
TargetCytokeratin 14
HostMouse
CloneLL002
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
Cytokeratin 14, a member of the intermediate filament family of keratins, is a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. [taken from NCBI Entrez Gene (Gene ID: 3861)].
Specifications
Clonality
Recombinant Monoclonal
Clone
LL002
Host
Mouse
Immunogen
Human Cytokeratin 14 (C terminal)
Isotype
IgG2a
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCytokeratin 14
Antigen Species
Human
Gene ID3861
Gene Symbol
KRT14
Gene Name
keratin 14
Uniprot IDP02533
Protein Name
Keratin, type I cytoskeletal 14
Alternate Names
CK14 • CK-14 • cytokeratin 14 • Cytokeratin-14 • EBS3 • EBS4 • K14 • keratin 14, type I • keratin, type I cytoskeletal 14 • Keratin-14 • NFJ
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was manufactured using variable regions (VH and VL) derived from the hybridoma.
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide corresponding to Human Cytokeratin 14 (C terminal).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-293 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-293 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-293 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-293Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
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