Bethyl Laboratories
Goat anti-Mouse IgA Heavy Chain Antibody Affinity Purified
Secondary
Catalog #A90-103A
TargetMouse IgA Heavy Chain
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
Product Description
IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum. Also exists in a secretory form (in mucosal regions) vs. serum IgA, which is found in serum.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgA. Cross reactivity to mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3 and IgE is less than 1%. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A90-103AOpen
Product DatasheetPDF · A90-103A · Lot 260929 (current lot)Open
Safety Data SheetPDF · A90-103AOpen
Product DatasheetPDF · A90-103A · Lot 24Open
Safety Data SheetPDF · A90-103AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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