Bethyl Laboratories
Mouse anti-Myoglobin Monoclonal Antibody [5mb-64]
Primary
Catalog #A500-045A
TargetMyoglobin
HostMouse
Clone5mb-64
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
Myoglobin is a protein responsible for binding both oxygen and iron, and is typically localized to heart and skeletal muscle tissue in mammals.
Specifications
Clonality
Monoclonal
Clone
5mb-64
Host
Mouse
Immunogen
Human Myoglobin
Isotype
IgG1
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetMyoglobin
Antigen Species
Human
Gene ID4151
Gene Symbol
MB
Gene Name
myoglobin
Uniprot IDP02144
Protein Name
Myoglobin
Alternate Names
myoglobgin • myoglobin • PVALB
Product Information
Applications
IHC, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Monoclonal antibody was purified from cell culture supernatant.
Immunogen was human Myoglobin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Western Blot (WB)1:1,000
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A500-045A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-045A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-045A · Lot 1 (current lot)Open
Safety Data SheetPDF · A500-045AOpen
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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