Bethyl Laboratories
Goat anti-Mouse IgG2c Cross-Adsorbed Antibody Affinity Purified
Secondary
Catalog #A90-236A
TargetMouse IgG2c
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
local_shipping
Order within 47 minutes for shipment by Tuesday, November 3
Product Description
Mouse IgG2c is generally regarded as the most active IgG subclass, as it can interact with all mouse activating Fc receptors.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2c was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2c. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2c was detected. This antibody may cross react with IgG2c from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A90-236AOpen
Product DatasheetPDF · A90-236A · Lot 1 (current lot)Open
Safety Data SheetPDF · A90-236AOpen
Product DatasheetPDF · A90-236A · Lot 4Open
Safety Data SheetPDF · A90-236AOpen
Product DatasheetPDF · A90-236A · Lot 1 (current lot)Open
Safety Data SheetPDF · A90-236AOpen
Product DatasheetPDF · A90-236A · Lot 12Open
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1