Goat anti-Mouse IgG2c Heavy Chain Antibody Affinity Purified

Specifications

Production & Epitope

Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c; sometimes referred to as the Igh 1b allele of IgG2a. (Martin et al., Journal of Immunological Methods, 1998, 212, 187-192)

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.

Applications

Not all listed applications have been specifically tested by our laboratory.