Bethyl Laboratories
Mouse anti-p16INK4a Recombinant Monoclonal Antibody [BC42]
Primary
Catalog #A700-292
Targetp16INK4a
HostMouse
CloneBC42
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, WB
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Product Description
p16INK4a is a negative regulator of CDK4 and CDK6 kinase activity and cell cycle progression. p16INK4a regulates the cell cycle by inhibiting cyclin/CDK complex formation and the phosphorylation of the retinoblastoma protein (pRb). The gene which encodes p16INK4a, CDKN2A, is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. The CDKN2A gene also generates a distinct transcript variant and protein, p14ARF, from an alternate open reading frame (ARF).
Specifications
Clonality
Recombinant Monoclonal
Clone
BC42
Host
Mouse
Immunogen
Human p16 INK4A
Isotype
IgG1
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
Targetp16INK4a
Antigen Species
Human
Gene ID1029
Gene Symbol
CDKN2A
Gene Name
cyclin dependent kinase inhibitor 2A
Uniprot IDP42771
Protein Name
Cyclin-dependent kinase inhibitor 2A
Alternate Names
alternative reading frame • ARF • CDK4 inhibitor p16-INK4 • CDK4I • CDKN2 • cell cycle negative regulator beta • CMM2 • cyclin-dependent kinase 4 inhibitor A • cyclin-dependent kinase inhibitor 2A • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) • INK4 • INK4A • MLM • MTS1 • MTS-1 • multiple tumor suppressor 1 • P14 • P14ARF • P16 • P16INK4 • p16-INK4 • P16INK4A • P16-INK4A • P19 • P19ARF • TP16
Product Information
Applications
Flow Cyt, ICC, IHC, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was manufactured using variable regions (VH and VL) derived from the hybridoma.
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein corresponding to human p16 INK4A.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-292 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-292 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-292 · Lot 230829Open
Safety Data SheetPDF · A700-292Open
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Resources
Standard Western Blot Protocol
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