Bethyl Laboratories
Goat anti-Mouse IgG1 Heavy Chain Antibody Affinity Purified
Secondary
Catalog #A90-105A
TargetMouse IgG1 Heavy Chain
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
local_shipping
Order within 4 hours and 16 minutes for shipment by Tuesday, November 17
Product Description
IgG1 is the most dominant subclass of IgG and is known to be able to cross the placenta, bind strongly to Fc receptors, and activate the complement pathway efficiently.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is less than 1%. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A90-105AOpen
Product DatasheetPDF · A90-105A · Lot 23 (current lot)Open
Safety Data SheetPDF · A90-105AOpen
Product DatasheetPDF · A90-105A · Lot 19Open
Safety Data SheetPDF · A90-105AOpen
Product DatasheetPDF · A90-105A · Lot 16Open
Safety Data SheetPDF · A90-105AOpen
Product DatasheetPDF · A90-105A · Lot 24Open
Safety Data SheetPDF · A90-105AOpen
Citations / Publications
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1