Bethyl Laboratories
Goat anti-Mouse IgG3 Heavy Chain Antibody Affinity Purified
Secondary
Catalog #A90-111A
TargetMouse IgG3 Heavy Chain
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG3 is a minor class of IgG found in the serum, but has a unique hinge region and contains more glycosylation sites than other IgG molecules, which make increase its potency within the immune system.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity to mouse IgM, IgA, IgG1, IgG2a, IgG2b, IgG2c, and IgE is less than 1%. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A90-111AOpen
Product DatasheetPDF · A90-111A · Lot 250325 (current lot)Open
Safety Data SheetPDF · A90-111AOpen
Product DatasheetPDF · A90-111A · Lot 12Open
Safety Data SheetPDF · A90-111AOpen
Product DatasheetPDF · A90-111A · Lot 13Open
Safety Data SheetPDF · A90-111AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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