Bethyl Laboratories
Goat anti-Mouse IgG2b Cross-Adsorbed Antibody Affinity Purified
Secondary
Catalog #A90-209A
TargetMouse IgG2b
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG2b structure is more restricted than the traditional Y-shaped IgG2a molecule. 2b tends to be shaped more like a T as a result of intramolecular disulfide interactions.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2b was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2b was detected. This antibody may cross react with IgG2b from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A90-209AOpen
Product DatasheetPDF · A90-209A · Lot 2 (current lot)Open
Safety Data SheetPDF · A90-209AOpen
Product DatasheetPDF · A90-209A · Lot 9Open
Safety Data SheetPDF · A90-209AOpen
Product DatasheetPDF · A90-209A · Lot 10Open
Safety Data SheetPDF · A90-209AOpen
Product DatasheetPDF · A90-209A · Lot 16Open
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