Bethyl Laboratories
Goat anti-Mouse IgG+IgM+IgA Heavy and Light Chain Antibody Affinity Purified
Secondary
Catalog #A90-144A
TargetMouse IgG+IgM+IgA Heavy and Light Chain
HostGoat
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG is the primary immunoglobulin protein found in blood/plasma and functions to neutralize toxins and cell-mediated cytotoxicity. IgM is typically referred to as a pentamer, and is found primarily in serum and is the first part of the antibody response to infection. IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was isolated by affinity chromatography using antigens coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG, IgA and IgM and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A90-144AOpen
Product DatasheetPDF · A90-144A · Lot 260720 (current lot)Open
Safety Data SheetPDF · A90-144AOpen
Product DatasheetPDF · A90-144A · Lot 6Open
Safety Data SheetPDF · A90-144AOpen
Product DatasheetPDF · A90-144A · Lot 8Open
Safety Data SheetPDF · A90-144AOpen
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