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Goat anti-Mouse IgM Heavy Chain Cross-Adsorbed Antibody FITC Conjugated

bethyl
Bethyl Laboratories Catalog #
Target:
Mouse IgM Heavy Chain
Reactivity:
Mouse
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin, DyLight® 488, DyLight® 550, DyLight® 594, DyLight® 650, DyLight® 680, DyLight® 755, DyLight® 800, FITC, HRP, Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)

Biotin (0.5 mg)

DyLight® 488 (0.5 mg)

DyLight® 550 (0.5 mg)

DyLight® 594 (0.5 mg)

DyLight® 650 (0.5 mg)

DyLight® 680 (0.5 mg)

DyLight® 755 (0.5 mg)

DyLight® 800 (0.5 mg)

FITC (0.5 mg)

HRP (0.5 mg)

$106.00 $174.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse
Antigen Species
Mouse
Minimal Reactivity
Human, Rat
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 680.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 800.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 755.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgM was detected. This antibody may cross react with IgM from other species.
Country of Origin
USA

Additional Product Information

IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.

Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 680 is excited at 682 (in PBS) and emits at 715 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 800 is excited at 770 (in PBS) and emits at 794 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 755 is excited at 754 (in PBS) and emits at 776 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.