Bethyl Laboratories

Mouse anti-Sodium Potassium ATPase alpha-1 Monoclonal Antibody [464.6]

Monoclonal Antibodies
Catalog #A500-031A
TargetSodium Potassium ATPase alpha-1
HostMouse
Clone464.6
ReactivityMouse, Human
ApplicationICC, IHC, IP, WB
Detection of human Sodium Potassium ATPase alpha-1 by western blot.
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Product Description

Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). [taken from NCBI Entrez Gene (Gene ID: 476)].

Specifications

Clonality
Monoclonal
Clone
464.6
Host
Mouse
Immunogen
Full length protein
Isotype
IgG1
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
Antigen Species
Human
Gene ID476
Gene Symbol
ATP1A1
Gene Name
ATPase Na+/K+ transporting subunit alpha 1
Uniprot IDP05023
Protein Name
Sodium/potassium-transporting ATPase subunit alpha-1
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Monoclonal antibody was purified from cell culture supernatant.

Immunogen was full length native protein, purified.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).

Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000

Documents

Product DatasheetPDF · A500-031A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-031A · Lot 1 (current lot)Open
Safety Data SheetPDF · A500-031AOpen
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