Bethyl Laboratories
Rabbit anti-Sp1 Antibody Affinity Purified
Primary
Catalog #A300-133A
TargetSp1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Specificity protein 1 (Sp1) is the original member of the Sp1-like/KLF family of zinc-finger transcription factors that binds GC-rich DNA elements to regulate transcription of genes involved in a variety of cellular functions. Sp-1 interacts with coactivators such as p300/CBP, TAFII130, and CRSP.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 700 and 750
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSp1
Antigen Species
Human
Gene ID6667
Gene Symbol
SP1
Gene Name
Sp1 transcription factor
Uniprot IDP08047
Protein Name
Transcription factor Sp1
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using the antigen immobilized on solid support.
The epitope recognized by A300-133A maps to a region between residues 700 and 740 using the numbering given in entry XP_028606.4 (GeneID 6667).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A300-133A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-133A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-133AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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