Bethyl Laboratories
Rabbit anti-B7-H3/CD276 Antibody Affinity Purified
Primary
Catalog #A304-368A
TargetB7-H3/CD276
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
CD276 may participate in the regulation of T-cell-mediated immune response. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It may also be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces and could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5ZPR3].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 484 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetB7-H3/CD276
Antigen Species
Human
Gene ID80381
Gene Symbol
CD276
Gene Name
CD276 molecule
Uniprot IDQ5ZPR3
Protein Name
CD276 antigen
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to B7-H3/CD276 immobilized on solid support.
The epitope recognized by A304-368A maps to a region between residue 484 to 534 of human CD276 Molecule using the numbering given in entry NP_001019907.1 (GeneID 80381).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A304-368A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-368A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-368AOpen
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