Bethyl Laboratories
Rabbit anti-CDK2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00374
TargetCDK2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Cyclin-dependent kinase 2 (CDK2) is a member of the CDK family of protein kinases that regulate cell cycle progression. CDK2 associates with cyclin E or cyclin A to drive the cell cycle through G1- and S-phase. Substrates of CDK2 include pRb, p53, p27, E2F, Brca1, CDK7, B-Myb, PP1 alpha, RNA Pol II CTD, axin, p220 NPAT, ribonucleotide reductase M2, Myo-D, MARCKS, HsCdc6, and CP110.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 248 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCDK2
Antigen Species
Human
Gene ID1017
Gene Symbol
CDK2
Gene Name
cyclin dependent kinase 2
Uniprot IDP24941
Protein Name
Cyclin-dependent kinase 2
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDK2 immobilized on solid support.
The epitope recognized by IHC-00374 maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00374Open
Product DatasheetPDF · IHC-00374 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00374 · Lot 1 (current lot)Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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