Bethyl Laboratories
Rabbit anti-RbBP5 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00172
TargetRbBP5
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Retinoblastoma binding protein 5 (RbBP5) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. RbBP5 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, ASH2L,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX. RbBP5 is also one of many proteins that bind the Retinoblastoma tumor suppressor protein, pRb.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 488 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetRbBP5
Antigen Species
Human
Gene ID5929
Gene Symbol
RBBP5
Gene Name
RB binding protein 5, histone lysine methyltransferase complex subunit
Uniprot IDQ15291
Protein Name
Retinoblastoma-binding protein 5
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RbBP5 immobilized on solid support.
The epitope recognized by IHC-00172 maps to a region between residues 500 and the C-terminus (residue 538) of human retinoblastoma binding protein 5 using the numbering given in entry NP_005048.2 (GeneID 5929).
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00172Open
Product DatasheetPDF · IHC-00172 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00172 · Lot 1 (current lot)Open
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