Bethyl Laboratories

Rabbit anti-SARS-CoV-2 Spike RBD Antibody Affinity Purified

Primary
Catalog #A170-100A
TargetSARS-CoV-2 Spike RBD
HostRabbit
ClonalityPolyclonal
ReactivitySpecies Independent
ApplicationWB
Detection of coronavirus spike protein RBD by western blot.
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Product Description

The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and mediates attachment of the virus particle and entry into the host cell though an interaction with the virla receptor binding domain (RBD) and ACE2. The spike protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. (NCBI)

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
HEK293-derived SARS CoV-2 S-RBD protein Ala319-Phe541, with a C-terminal HIS6 tag
Isotype
IgG
Reactivity
Species Independent
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Target Identity
Antigen Species
SARS-CoV-2
Gene ID43740568
Gene Symbol
S
Gene Name
surface glycoprotein
Uniprot IDP0DTC2
Protein Name
Spike glycoprotein
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Immunogen was HEK293-derived SARS CoV-2 S-RBD protein fragment (Ala319-Phe541) using the numbering given in entry YP_009724390.1.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Not all listed applications have been specifically tested by our laboratory.

Western Blot (WB)1:10,000 - 1:25,000

Documents

Safety Data SheetPDF · A170-100AOpen
Product DatasheetPDF · A170-100A · Lot 1 (current lot)Open
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