Bethyl Laboratories
Mouse anti-CD8 alpha Monoclonal Antibody Purified [C8/144B]
Monoclonal Antibodies
Catalog #A500-021A
TargetCD8 alpha
HostMouse
CloneC8/144B
ReactivityHuman
ApplicationFlow Cyt, IHC, IHC-IF
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Product Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].
Specifications
Clonality
Monoclonal
Clone
C8/144B
Host
Mouse
Immunogen
A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule
Isotype
IgG1
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetCD8 alpha
Antigen Species
Human
Gene ID925
Gene Symbol
CD8A
Gene Name
CD8a molecule
Uniprot IDP01732
Protein Name
T-cell surface glycoprotein CD8 alpha chain
Product Information
Applications
Flow Cyt, IHC, IHC-IF
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was purified from cell culture supernatant.
Immunogen was a 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule.
Applications
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 50% methanol. 2.5 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A500-021A · Lot 3 (current lot)Open
Product DatasheetPDF · A500-021A · Lot 3 (current lot)Open
Product DatasheetPDF · A500-021A · Lot 3 (current lot)Open
Safety Data SheetPDF · A500-021AOpen
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Resources
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