Bethyl Laboratories
Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody [BLR044F]
Monoclonal Antibodies
Catalog #A700-044
TargetCD8 alpha
HostRabbit
CloneBLR044F
ReactivityHuman
ApplicationFlow Cyt, IHC, IHC-IF, mIF, WB
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Product Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR044F
Host
Rabbit
Immunogen
Between 185 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetCD8 alpha
Antigen Species
Human
Gene ID925
Gene Symbol
CD8A
Gene Name
CD8a molecule
Uniprot IDP01732
Protein Name
T-cell surface glycoprotein CD8 alpha chain
Product Information
Applications
Flow Cyt, IHC, IHC-IF, mIF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 185 and the C-terminus (residue 235) of human T-cell surface glycoprotein CD8 alpha chain using the numbering given in entry NP_001759.3 (Gene ID 925)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-044 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-044 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-044 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-044Open
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
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