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Goat anti-Rat IgM Heavy Chain Antibody Biotinylated

bethyl
Bethyl Laboratories

Catalog #

Target:

Rat IgM Heavy Chain

Reactivity:

Rat

Host:

Goat

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Biotin

,

DyLight® 488

,

DyLight® 550

,

DyLight® 594

,

DyLight® 650

,

FITC

,

HRP

,

Unconjugated

Purity:

Antigen Affinity Purified

Unconjugated (1 mg)

Biotin (1 mg)

DyLight® 488 (0.5 mg)

DyLight® 550 (0.5 mg)

DyLight® 594 (0.5 mg)

DyLight® 650 (0.5 mg)

FITC (1 mg)

HRP (1 mg)

Product Details

Specifications
Verified Reactivity

Rat

Antigen Species

Rat

Concentration
1 mg/ml 0.5 mg/ml
Fluorophore/Protein
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt,

2 years from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide


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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400


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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


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Production Procedures

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. This antibody may cross react with IgM from other species.

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. This antibody may cross react with IgM from other species.

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. This antibody may cross react with IgM from other species.

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. This antibody may cross react with IgM from other species.

Additional Product Information

IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.

Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.