Western Blot (WB) Support

If you are experiencing difficulties with a Bethyl product, please first consult the Frequently Asked Questions below. If your question isn't answered, please complete the form as accurately as possible. The information is helpful to troubleshoot the differences between the observed results and the expected results. Once submitted, a Technical Support representative will contact you within 1 business day. Please note that all comments and suggestions are taken seriously by Bethyl Laboratories.

Western Blot Support FAQs

  • The sequences of the immunogens are proprietary. All of the data sheets on our polyclonal and monoclonal antibodies provide a range of amino acids in the protein to which the peptide maps.

    1. The migration patterns of molecular weight markers can differ between vendors.
    2. The type of gel and buffer system used can have effects on protein mobility.



Western Blot Technical Support Form

Customer Information












Product Information







Sample
Cell Line or Tissue



Lysis Buffer




Lysate


Electrophoresis
Gel




Transfer



Western Blotting Procedure
Blocking


Primary Antibody




Secondary Antibody








Detection Method
Controls


Replicates











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