NEW!

Goat anti-Rabbit IgG-Fc Fragment Antibody Biotinylated

bethyl
Bethyl Laboratories

Catalog #

Target:

Rabbit IgG-Fc Fragment

Reactivity:

Rabbit

Host:

Goat

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Biotin

,

DyLight® 488

,

DyLight® 550

,

DyLight® 594

,

DyLight® 650

,

FITC

,

HRP

,

Unconjugated

Purity:

Antigen Affinity Purified

,

Antiserum

Antiserum (2 ml)

Unconjugated (1 mg)

Biotin (1 mg)

DyLight® 488 (0.5 mg)

DyLight® 550 (0.5 mg)

DyLight® 594 (0.5 mg)

DyLight® 650 (0.5 mg)

FITC (1 mg)

HRP (1 mg)

Product Details

Specifications
Verified Reactivity

Rabbit

Antigen Species

Rabbit

Concentration
1 mg/ml 0.5 mg/ml
Fluorophore/Protein
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt,

2 years from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide


Request Formulation Change

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400


Request Formulation Change

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


Request Formulation Change

Serum containing 0.09% Sodium Azide


Request Formulation Change
Production Procedures

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis, this antibody reacts specifically with rabbit IgG. This antibody may cross react with IgG from other species.

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis, this antibody reacts specifically with rabbit IgG. This antibody may cross react with IgG from other species.

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis, this antibody reacts specifically with rabbit IgG. This antibody may cross react with IgG from other species.

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis, this antibody reacts specifically with rabbit IgG. This antibody may cross react with IgG from other species.

Additional Product Information

IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.

Applications

Not all listed applications have been specifically tested by our laboratory. For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).

Optimal working dilutions should be determined experimentally by the investigator. DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.