/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Myc Tag Recombinant Monoclonal Antibody [BLRE01G]
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Primary Antibodies  

Rabbit anti-Myc Tag Recombinant Monoclonal Antibody [BLRE01G]

bethyl
Bethyl Laboratories

Catalog # A191-101

Validated
Target:

Myc Tag

Reactivity:

Human

Applications:

ELISA

,

ICC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLRE01G

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

Product Details

Specifications
Verified Reactivity

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

The GST tag is derived from glutathione S-transferase. Its affinity for glutathione makes it ideal for protein purification purposes, but can also be used for WB, IP, and microscopic purposes. The tag is larger than most epitope tags and contributes to solubility of the tagged protein. Further, the tag can also be removed via a proteolytic cleavage if needed.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.