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Primary Antibodies  

Rabbit anti-GFP Recombinant Monoclonal Antibody [BLRE04L]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog # A191-104

Validated
Target:

GFP

Applications:

ICC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

GFP

Format:

Whole IgG

Immunogen:

Green Fluorescent Protein

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

Product Details

Specifications
Concentration
50 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Green fluorescent protein (GFP) and variants such as EGFP, YP, CFP, AcGFP, or BFP are widely used as fluorescent markers of gene expression and for determination of protein localization and motility in living cells.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.