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Primary Antibodies  

Rabbit anti-MAD2 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (25)
Target:

MAD2

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 100 and 150

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

MAD2 (Mitotic Arrest Deficiency 2) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD2 is a subunit of the MCC complex that includes BubR1, Bub3, Cdc20 and Mad2, which may function to inhibit the APC/c (Anaphase Promoting Complex/Cyclosome). MAD2 is regulated by MAD1.

4085

MAD2L1

mitotic arrest deficient 2 like 1

Q13257

Mitotic spindle assembly checkpoint protein MAD2A

Alternate Names

HSMAD2; MAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2 mitotic arrest deficient-like 1; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; mitotic spindle assembly checkpoint protein MAD2A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.