/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NCOA5/CIA Antibody
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Primary Antibodies  

Rabbit anti-NCOA5/CIA Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

NCOA5/CIA

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 525 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

NCOA5 (Nuclear Receptor Coactivator 5), also known as CIA (Coactivator Independent of AF-2 function), has been shown interact exclusively with estrogen receptor alpha (ERalpha) and beta (ERbeta) to function as a coactivator and enhance transcriptional activation by ER. NCOA5 has also been demonstrated to function as a corepressor of MYC/c-myc in response to 17-beta estradiol.

57727

NCOA5

nuclear receptor coactivator 5

Q9HCD5

Nuclear receptor coactivator 5

Alternate Names

bA465L10.6; CIA; coactivator independent of AF-2; NCoA-5; nuclear receptor coactivator 5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.