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Primary Antibodies  

Rabbit anti-DOT1L Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (10)
Target:

DOT1L

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1485 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.

84444

DOT1L

DOT1 like histone lysine methyltransferase

Q8TEK3

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Alternate Names

DOT1; DOT1 like histone H3K79 methyltransferase; DOT1-like histone methyltransferase; DOT1-like protein; DOT1-like, histone H3 methyltransferase; H3-K79-HMTase; histone H3-K79 methyltransferase; histone methyltransferase DOT1L; histone-lysine N-methyltransferase, H3 lysine-79 specific; KMT4; lysine N-methyltransferase 4

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.