/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-BIG1/ARFGEF1 Antibody
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Primary Antibodies  

Rabbit anti-BIG1/ARFGEF1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (8)
Target:

BIG1/ARFGEF1

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1800 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

BIG1/ARFGEF1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.

10565

ARFGEF1

ADP ribosylation factor guanine nucleotide exchange factor 1

Q9Y6D6

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Alternate Names

ADP-ribosylation factor guanine nucleotide-exchange factor 1; ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited); ARFGEP1; BIG1; Brefeldin A-inhibited GEP 1; brefeldin A-inhibited guanine nucleotide-exchange protein 1; P200; p200 ARF guanine nucleotide exchange factor; p200 ARF-GEP1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.