Rabbit anti-CEP170 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CEP170

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1534 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to CEP170 immobilized on solid support.

The epitope recognized by A301-024A maps to a region between residue 1534 and the C-teriminus (residue 1584) of human centrosomal protein 170kDa using the numbering given in entry NP_055627.2 (GeneID 9859).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to CEP170 immobilized on solid support.

The epitope recognized by A301-024A-T maps to a region between residue 1534 and the C-teriminus (residue 1584) of human centrosomal protein 170kDa using the numbering given in entry NP_055627.2 (GeneID 9859).

Additional Product Information

CEP170 (centrosomal protein 170 kDa) was identified as an interaction partner of polo-like kinase 1 (Plk1), a serine/threonine kinase involved in spindle formation and chromosome segregation. CEP170 is a forkhead-associated domain protein that localizes to the centrosomes and functions to maintain microtubule organization.

9859

CEP170

centrosomal protein 170

Q5SW79

Centrosomal protein of 170 kDa

Alternate Names

centrosomal protein 170kDa; centrosomal protein of 170 kDa; Cep170; FAM68A; KAB; KARP-1-binding protein; KARP1-binding protein; KIAA0470; XRCC5 binding protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.