/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-AktS1/PRAS40 Antibody
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Primary Antibodies  

Rabbit anti-AktS1/PRAS40 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

AktS1/PRAS40

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 225 and 256

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

AktS1/PRAS40 interacts with raptor as a component of the mammalian target of rapamycin complex 1 (mTORC1). One study suggests that AktS1/PRAS40 may function to regulate mTORC1 kinase activity by inhibiting substrate binding. Another study suggests that AktS1/PRAS40 functions downstream of mTORC1 and upstream from its effectors S6K1, and 4E-BP1.

84335

AKT1S1

AKT1 substrate 1

Q96B36

Proline-rich AKT1 substrate 1

Alternate Names

40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline rich); Lobe; PRAS40; proline-rich AKT1 substrate 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.