/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ILK Antibody
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Primary Antibodies  

Rabbit anti-ILK Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

ILK

Reactivity:

Human

,

Mouse

Applications:

ICC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 402 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Guinea Pig,

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ILK immobilized on solid support.

The epitope recognized by A301-258A maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ILK immobilized on solid support.

The epitope recognized by A301-258A-T maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).

Additional Product Information

Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.

3611

ILK

integrin linked kinase

Q13418

Integrin-linked protein kinase

Alternate Names

59 kDa serine/threonine-protein kinase; epididymis secretory protein Li 28; HEL-S-28; ILK-1; ILK-2; integrin-linked kinase-2; integrin-linked protein kinase; P59; p59ILK

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.