/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ANKIB1 Antibody
Primary Antibodies  

Rabbit anti-ANKIB1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

ANKIB1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1039 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ANKIB1 immobilized on solid support.

The epitope recognized by A301-368A maps to a region between residue 1039 and 1089 of human ankyrin repeat and IBR domain-containing protein 1 using the numbering given in entry NP_061877.1 (GeneID 54467).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ANKIB1 immobilized on solid support.

The epitope recognized by A301-368A-T maps to a region between residue 1039 and 1089 of human ankyrin repeat and IBR domain-containing protein 1 using the numbering given in entry NP_061877.1 (GeneID 54467).

Additional Product Information

The function of Ankyrin repeat and IBR domain-containing protein 1 (ANKIB1) has not been characterized. Structurally, ANKIB1 contains two ankyrin repeats, one IBR-type zinc finger, two RING-type zinc fingers, and an ubiquitin-interacting motif (UIM) repeat. The presence of a UIM suggests that ANKIB1 may have a function in the metabolism of ubiquitinated proteins.

54467

ANKIB1

ankyrin repeat and IBR domain containing 1

Q9P2G1

Ankyrin repeat and IBR domain-containing protein 1

Alternate Names

ankyrin repeat and IBR domain-containing protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.