/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-AKAP12/Gravin Antibody
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Primary Antibodies  

Rabbit anti-AKAP12/Gravin Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

AKAP12/Gravin

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1732 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to AKAP12/Gravin immobilized on solid support.

The epitope recognized by A301-402A maps to a region between residue 1731 and 1781 of human A kinase (PRKA) anchor protein (gravin) 12 using the numbering given in entry AAC51366.1 (GeneID 9590).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to AKAP12/Gravin immobilized on solid support.

The epitope recognized by A301-402A-T maps to a region between residue 1731 and 1781 of human A kinase (PRKA) anchor protein (gravin) 12 using the numbering given in entry AAC51366.1 (GeneID 9590).

Additional Product Information

AKAP12/Gravin is a tumor suppressor protein belonging to the A-kinase anchoring protein family. A-kinase anchoring proteins function as molecular scaffolds for the subcellular compartmentalization of A- kinase signaling molecules. In addition to its role in cell signaling, AKAP12/Gravin has also been identified as an autoantigen in some cases of myasthenia gravis and has recently been shown to play a role in leukemogenesis.

9590

AKAP12

A-kinase anchoring protein 12

Q02952

A-kinase anchor protein 12

Alternate Names

A kinase (PRKA) anchor protein 12; AKAP 250; AKAP-12; AKAP250; A-kinase anchor protein 12; A-kinase anchor protein 250 kDa; A-kinase anchor protein, 250kDa; Gravin; kinase scaffold protein gravin; Myasthenia gravis autoantigen; myasthenia gravis autoantigen gravin; Src-Suppressed C Kinase Substrate; SSeCKS

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.