/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-KANK2/SIP Antibody
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Primary Antibodies  

Rabbit anti-KANK2/SIP Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

KANK2/SIP

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 625 and 675

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to KANK2/SIP immobilized on solid support.

The epitope recognized by A301-408A-T maps to a region between residue 625 and 675 of human KN motif and ankyrin repeat domains 2 (SRC-1 interacting protein) using the numbering given in entry NP_056308.2 (GeneID 25959).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to KANK2/SIP immobilized on solid support.

The epitope recognized by A301-408A maps to a region between residue 625 and 675 of human KN motif and ankyrin repeat domains 2 (SRC-1 interacting protein) using the numbering given in entry NP_056308.2 (GeneID 25959).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

KANK2/SIP was first identified during a genome wide-screen for proteins that interact with the SRC transcriptional coactivator. KANK2/SIP was also identified by domain and phylogenetic analysis to be a member of the KANK ankyrin-repeat domain-containing protein family. KANK related proteins possess a conserved ankyrin-repeat and coiled-coil domain. KANK2, as well as other KANK proteins, appear to play a role in the formation of actin stress fibers.

25959

KANK2

KN motif and ankyrin repeat domains 2

Q63ZY3

KN motif and ankyrin repeat domain-containing protein 2

Alternate Names

ANKRD25; ankyrin repeat domain-containing protein 25; kidney ankyrin repeat-containing protein 2; KN motif and ankyrin repeat domain-containing protein 2; matrix-remodeling-associated protein 3; MXRA3; NPHS16; PPKWH; SIP; SRC-1-interacting protein; SRC1-interacting protein; SRC-interacting protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.