/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CEP131/AZ1 Antibody
Primary Antibodies  

Rabbit anti-CEP131/AZ1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (7)
Target:

CEP131/AZ1

Reactivity:

Human

Applications:

ICC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to CEP131/AZ1 immobilized on solid support.

The epitope recognized by A301-415A maps to a region between residue 1 and 50 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to CEP131/AZ1 immobilized on solid support.

The epitope recognized by A301-415A-T maps to a region between residue 1 and 50 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).

Additional Product Information

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

22994

CEP131

centrosomal protein 131

Q9UPN4

Centrosomal protein of 131 kDa

Alternate Names

5-azacytidine induced 1; 5-azacytidine-induced protein 1; AZ1; AZI1; centrosomal protein 131 kDa; centrosomal protein 131kDa; centrosomal protein of 131 kDa; pre-acrosome localization protein 1; ZA1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.