/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NFRKB Antibody
Primary Antibodies  

Rabbit anti-NFRKB Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

NFRKB

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1025 and 1075

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to NFRKB immobilized on solid support.

The epitope recognized by A301-459A maps to a region between residue 1025 and 1075 of human nuclear factor related to kappaB binding protein using the numbering given in entry AAH63280.1 (GeneID 4798).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to NFRKB immobilized on solid support.

The epitope recognized by A301-459A-T maps to a region between residue 1025 and 1075 of human nuclear factor related to kappaB binding protein using the numbering given in entry AAH63280.1 (GeneID 4798).

Additional Product Information

NFRKB (nuclear factor related to kappa-B-binding protein) was first identified as a factor that could bind a variant NF-kappa B site in the interleukin-2 receptor alpha (IL-2R alpha) gene. NFRKB appears to be a transcription factor central to immune cell development. Evidence of this comes from the identification of NFRKB as an amplified gene in several cases of acute myeloid leukemia. Additionally, in another clinical case, a partial deletion of the chromosomal region that includes NFRKB is shown to be associated with combined cellular immunodeficiency.

4798

NFRKB

nuclear factor related to kappaB binding protein

Q6P4R8

Nuclear factor related to kappa-B-binding protein

Alternate Names

DNA-binding protein R kappa B; DNA-binding protein R kappa-B; INO80 complex subunit G; INO80G; nuclear factor related to kappa-B-binding protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.