/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Astrin/MAP126 Antibody
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Primary Antibodies  

Rabbit anti-Astrin/MAP126 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

Astrin/MAP126

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Astrin/MAP126 has been identified as a mitotic spindle-associated protein essential for centrosome integrity and the progression through mitosis. Astrin/MAP126 interacts with Ninein and Aurora-A at the centrosome. Ninein, but not Aurora-A, appears to be required for proper localization of Astrin/MAP126 to the centrosome during S and G2 phase. A pool of Astrin/MAP126 also localizes to the outer kinetochore where it plays an essential role in chromatid adhesion and chromosome congression and segregation. The localization of Astrin/MAP126 to the spindle pole and kinetochore has been shown to be dependent on its phosphorylation by glycogen synthase kinase 3beta (GSK3beta).

10615

SPAG5

sperm associated antigen 5

Q96R06

Sperm-associated antigen 5

Alternate Names

astrin; DEEPEST; hMAP126; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; Mitotic spindle-associated protein p126; sperm-associated antigen 5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.