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Primary Antibodies  

Rabbit anti-MBD4 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (4)
Target:

MBD4

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

MBD4 (methyl-CpG-binding domain protein 4) is a member of the MBD family of proteins that mainly functions to bind methylated CpG sites and to repress gene transcription. Unlike its family members, MBD4 exhibits thymine glycosylase activity and has been shown to play an important role in DNA mismatch repair and apoptosis.

8930

MBD4

methyl-CpG binding domain 4, DNA glycosylase

O95243

Methyl-CpG-binding domain protein 4

Alternate Names

3,N(4)-ethenocytosine glycosylase; G/5-fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; MED1; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 4; methyl-CpG-binding endonuclease 1; methyl-CpG-binding protein MBD4; mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.