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Rabbit anti-ROD/KNTC1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ROD/KNTC1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 375 and 425

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

KNTC1 (kinetochore-associated protein 1) is the human ortholog of the drosophila rough deal (ROD) protein. Defects in the drosophila ROD protein result in chromosome missegregation. Similarly, human ROD (KNTC1) is proposed to function as a mitotic checkpoint protein present in multiprotein complexes of the kinetochore.

9735

KNTC1

kinetochore associated 1

P50748

Kinetochore-associated protein 1

Alternate Names

hRod; HsROD; kinetochore-associated protein 1; ROD; Rough deal homolog; Rough Deal homolog, centromere/kinetochore protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.