/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-eIF3J/EIF3S1 Antibody
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Primary Antibodies  

Rabbit anti-eIF3J/EIF3S1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

eIF3J/EIF3S1

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 208 and 258

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Eukaryotic initiation factor 3 subunit J (eIF3J) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3J is proposed to act as a factor that further stabilizes the interaction between eIF3 and the ribosome.

8669

EIF3J

eukaryotic translation initiation factor 3 subunit J

O75822

Eukaryotic translation initiation factor 3 subunit J

Alternate Names

eIF3 p35; eIF-3-alpha; eIF3-alpha; eIF3j; eIF3-p35; EIF3S1; Eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3 subunit J; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.