/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NUP35 Antibody
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Primary Antibodies  

Rabbit anti-NUP35 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (5)
Target:

NUP35

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 276 and 376

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Human Nup35 is the 35 kDa ortholog of the yeast Nup53p. Nup35 is also referred to as Nup53 or vertebrate Nup53. Nup35 is a component of a NPC subcomplex that contains Nup35, Nup93, Nup155, and Nup205.

129401

NUP35

nucleoporin 35

Q8NFH5

Nucleoporin NUP53

Alternate Names

35 kDa nucleoporin; mitotic phosphoprotein 44; MP44; MP-44; NP44; nuclear pore complex protein Nup53; nucleoporin 35kDa; nucleoporin NUP35; nucleoporin NUP53; NUP53

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.