/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-WDFY3 Antibody
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Primary Antibodies  

Rabbit anti-WDFY3 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

WDFY3

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 500 and 550

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

WDFY3 (WD repeat and FYVE domain –containing protein 3) was identified in a screen for phosphatidylinositol -3-phosphate [PtdIns(3)P]- binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3)-binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery.

23001

WDFY3

WD repeat and FYVE domain containing 3

Q8IZQ1

WD repeat and FYVE domain-containing protein 3

Alternate Names

ALFY; autophagy-linked FYVE protein; BCHS; MCPH18; WD repeat and FYVE domain-containing protein 3; ZFYVE25

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.