/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MADD Antibody
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Primary Antibodies  

Rabbit anti-MADD Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

MADD

Reactivity:

Human

Applications:

IHC

,

IHC-IF

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1597 and 1647

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to MADD immobilized on solid support.

The epitope recognized by A302-144A-T maps to a region between residue 1597 and 1647 of human MAP-kinase activating death domain using the numbering given in entry NP_003673.3 (GeneID 8567).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to MADD immobilized on solid support.

The epitope recognized by A302-144A maps to a region between residue 1597 and 1647 of human MAP-kinase activating death domain using the numbering given in entry NP_003673.3 (GeneID 8567).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

MAP kinase-activating death domain protein (MADD) is a death domain containing protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activate protein kinase (MAPK) and transduce apoptotic signals. MADD is a multi-pass membrane protein that exists as multiple alternatively spliced forms.

8567

MADD

MAP kinase activating death domain

Q8WXG6

MAP kinase-activating death domain protein

Alternate Names

DENN; differentially expressed in normal and neoplastic cells; IG20; insulinoma glucagonoma clone 20; MAP kinase-activating death domain protein; Rab3 GDP/GTP exchange factor; RAB3GEP

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.