/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-BRD7 Antibody
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Primary Antibodies  

Rabbit anti-BRD7 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (6)
Target:

BRD7

Reactivity:

Human

Applications:

Flow Cyt

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 601 and 651

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to BRD7 immobilized on solid support.

The epitope recognized by A302-304A maps to a region between residue 601 and 651 of human bromodomain containing 7 using the numbering given in entry NP_037395.2 (GeneID 29117).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to BRD7 immobilized on solid support.

The epitope recognized by A302-304A-T maps to a region between residue 601 and 651 of human bromodomain containing 7 using the numbering given in entry NP_037395.2 (GeneID 29117).

Additional Product Information

Bromodomain-containing protein 7 was identified in a two-hybrid screen as an interactor of interferon regulatory factor-2 (IRF-2) and called CELTIX1. BRD7 was also identified as a binding partner of disheveled-1 (Dvl-1) and found to play a role in Wnt signaling and the regulation of glycogen synthase kinase 3-beta activity. Recently, BRD7 was identified as a subunit of the polybromo-associated BRG1-associated factor (PBAF) complex and shown to be required for the modulation of transcription in embryonic stem cells.

29117

BRD7

bromodomain containing 7

Q9NPI1

Bromodomain-containing protein 7

Alternate Names

75 kDa bromodomain protein; BP75; bromodomain-containing protein 7; CELTIX1; NAG4; protein CELTIX-1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.