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Primary Antibodies  

Rabbit anti-PAP-gamma Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

PAP-gamma

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 686 and 736

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Poly(A) polymerase gamma (PAP-gamma) is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. PAP-gamma is exclusively localized in the nucleus and exhibits nonspecific as well as CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity [taken from NCBI Entrez Gene (GeneID: 64895)].

64895

PAPOLG

poly(A) polymerase gamma

Q9BWT3

Poly(A) polymerase gamma

Alternate Names

neo-PAP; neo-poly(A) polymerase; nuclear poly(A) polymerase gamma; PAP-gamma; poly(A) polymerase gamma; polynucleotide adenylyltransferase gamma; signal recognition particle RNA-adenylating enzyme; SRP RNA 3' adenylating enzyme/pap2; SRP RNA 3'-adenylating enzyme; SRP RNA-adenylating enzyme

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.