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Rabbit anti-AP4 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

AP4

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 288 and 338

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

AP4 is a basic helix-loop-helix-zipper (bHLH-ZIP) transcription factor. BHLH-ZIP transcription factors contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG [taken from NCBI Entrez Gene (GeneID: 7023)].

7023

TFAP4

transcription factor AP-4

Q01664

Transcription factor AP-4

Alternate Names

activating enhancer-binding protein 4; AP-4; bHLHc41; class C basic helix-loop-helix protein 41; transcription factor AP-4; transcription factor AP-4 (activating enhancer binding protein 4)

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.