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Rabbit anti-PUF60 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

PUF60

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 400 and 450

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Poly-U binding splicing factor 60KDa (PUF60) is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 is implicated in the xeroderma pigmentosum disorder [taken from NCBI Entrez Gene (Gene ID: 22827)].

22827

PUF60

poly(U) binding splicing factor 60

Q9UHX1

Poly(U)-binding-splicing factor PUF60

Alternate Names

60 kDa poly(U)-binding-splicing factor; FBP interacting repressor; FBP-interacting repressor; FIR; FUSE-binding protein-interacting repressor; poly(U) binding splicing factor 60KDa; poly(U)-binding-splicing factor PUF60; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; RoBPI; Siah binding protein 1; Siah-binding protein 1; SIAHBP1; Siah-BP1; VRJS

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.